Glutamate Synthase(GOGAT) Activity Assay Kit
Cat.No:BC0075 Solarbio
Detection Method:Spectrophotometer/Microplate Reader
Storage:Store at -20℃,6 months
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Glutamate Synthase(GOGAT) Activity Assay KitDetection Method:Spectrophotometer/Microplate Reader
Storage:Store at -20℃,6 months
Qty:
Size:
Name | Glutamate Synthase(GOGAT) Activity Assay Kit |
Detection Method | Spectrophotometer/Microplate Reader |
Storage | Store at -20℃,6 months |
Recommend | If 96-well plate is used for determination, 96-well UV plate should be used, recommended item numberYA0602 |
Unit | Kit |
Specification | 100T/96S |
Determination of Significance:
Glutamine oxoglutarate aminotransferase (also known as Glutamate synthase) is an enzyme and frequently abbreviated as GOGAT. GOGAT is mainly found in the protoplasts of prokaryotes, yeasts and non-green tissues of higher plants. Together with glutamine synthetase (GS), it constitutes the GS/GOGAT cycle and participates in the regulation of ammonia assimilation.
Measurement Principle:
GOGAT uses NADH as an electron donor to catalyze the amino transfer of glutamine to α-ketoglutarate to form two molecules of glutamic acid. The activity of GOGAT can be determined by the decrease rate of NADH at 340 nm.
Self Provided:
Microplate reader/spectrophotometer, desk centrifuge, adjustable transferpettor, water bath, micro quartz cuvette/96 well UV flat-bottom plate, mortar/ homogenizer, ice, distilled water.
Note:Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.
Remark:These protocols are for reference only. Solarbio does not independently validate these methods.
Note:
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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