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Determination of Significance：
Glutamine oxoglutarate aminotransferase (also known as Glutamate synthase) is an enzyme and frequently abbreviated as GOGAT. GOGAT is mainly found in the protoplasts of prokaryotes, yeasts and non-green tissues of higher plants. Together with glutamine synthetase (GS), it constitutes the GS/GOGAT cycle and participates in the regulation of ammonia assimilation.
Measurement Principle：
GOGAT uses NADH as an electron donor to catalyze the amino transfer of glutamine to α-ketoglutarate to form two molecules of glutamic acid. The activity of GOGAT can be determined by the decrease rate of NADH at 340 nm.
Self Provided：
Microplate reader/spectrophotometer, desk centrifuge, adjustable transferpettor, water bath, micro quartz cuvette/96 well UV flat-bottom plate, mortar/ homogenizer, ice, distilled water.
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