Nitrate Reductase(NR) Activity Assay Kit
Cat.No：BC0085 Solarbio
Detection Method：Spectrophotometer/Microplate Reader
Storage：Store at -20℃，6 months
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> Biochemical Assay Kits > Nitrogen Metabolism Series > Other Nitrogen Metabolism Series > Nitrate Reductase(NR) Activity Assay Kit
Product Information
| Name | Nitrate Reductase(NR) Activity Assay Kit |
| Detection Method | Spectrophotometer/Microplate Reader |
| Storage | Store at -20℃，6 months |
| Recommend | If 96-well plate is used for determination, 96-well UV plate should be used, recommended item numberYA0602 |
| Unit | Kit |
| Specification | 100T/96S |
Determination of Significance：
NR (EC 1.7.1.3) is a key enzyme in the transformation of plant nitrate nitrogen into ammonia nitrogen as well as an induction enzyme, widely exists in plants and has an impact on crop yield and quality.
Measurement Principle：
NR catalyzed nitrate reduction to nitrite, with NO3ˉ + NADH + H＋→ NO2ˉ + NAD＋+ H2O. NADH has a characteristic absorption peak at 340 nm. The change of absorbance at 340 nm can indicate the enzyme activity.
Self Provided：
Table centrifuge, water-bath, micro quartz cuvette/96 well UV flat-bottom plate, spectrophotometer/ microplate reader, mortar/ homogenate, ice and distilled water.
Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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