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Determination of Significance：
Urease is an enzyme that catalyzes the hydrolysis of urea into carbon dioxide and ammonia. The microbial quantity of soil, organic matter content, total nitrogen and available nitrogen content have positive correlation with soil urease activity. Soil nitrogen status is determined by soil urease activity. The ammonia is determined by the indophenol blue method, resulting in blue indophenol produced is proportional to the concentration of ammonia.
Measurement Principle：
Urease is an enzyme that catalyzes the hydrolysis of urea into carbon dioxide and ammonia. The microbial quantity of soil, organic matter content, total nitrogen and available nitrogen content have positive correlation with soil urease activity. Soil nitrogen status is determined by soil urease activity. The ammonia is determined by the indophenol blue method, resulting in blue indophenol produced is proportional to the concentration of ammonia.
Self Provided：
Spectrophotometer/microplate reader, thermostat water bath, transferpettor, micro glass cuvette/ 96 well flat-bottom plate, ice, 30-50 mesh sieve (or smaller), distilled water, Methylbenzene (Express delivery not allowed) and distilled water.
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