Human CA125 ELISA Kit_Human_ELISA_Solarbio-Global Leader in Antibody and Elisa Kit

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SKU	Tongzhou Beijing	Haidian Beijing	Wuhan	Guangzhou
SEKH-0527-48T	Inquiry	Inquiry	Inquiry	Inquiry
SEKH-0527-96T	Inquiry	Inquiry	Inquiry	Inquiry

Chinese Name	人卵巢癌抗原CA125检测试剂盒
English Name	Human CA125 ELISA Kit
Synonyms	CA125 ovarian cancer antigen; CA-125; CA125MUC-16; FLJ14303; MUC16; mucin 16, cell surface associated; mucin-16; Ovarian cancer-related tumor marker CA125; Ovarian carcinoma antigen CA125
Storage	2-8℃，6 months
Detection Target	CA125
Sample Type	Serum，Plasma，Cell Culture Supernatant
Reactivity	Human
Application	ELISA
Reaction Type	Sandwich-ELISA
Quantitative/Qualitative	Quantitative
Detection Range	31.25-2000 pg/ml
Sensitivity	15.625 pg/ml
Specificity	Do not react with Human E-Selectin/FcChimera， Human Galectin-1， Human L-Selectin/FcChimera， Human Mesothelin， Human MUC-1， Human Siglec-9/FcChimera etc.
Precision	Both intra-plate and inter-plate variation coefficients were less than 10%
Recovery	80%-120%
Assay Time	3h
Swiss Prot	Q8WXI7
Gene ID	94025
Detection Principle	Solarbio ELISA试剂盒采用基于双抗体夹心法的酶联免疫吸附检测技术。将抗人CA125/MUC16单克隆抗体包被在酶标板上；分别加入梯度稀释的标准品和预稀释的样本，标准品和样本中的人CA125/MUC16会与酶标板上的包被抗体充分结合；洗板后加入生物素化抗人CA125/MUC16抗体，该抗体会与板子上包被抗体捕获的标准品和样本中的人CA125/MUC16发生特异性结合；洗板后加入辣根过氧化物酶（HRP）标记的链霉亲和素，生物素与链霉亲和素会发生高强度的非共价结合；洗板后加入显色剂底物TMB，若反应孔中样品存在不同浓度的人CA125/MUC16，则HRP会使无色TMB变成不同深浅（正相关）的蓝色物质，加入终止液后反应孔会变成黄色；最后，在λmax=450 nm（OD=450 nm）处测定反应孔样品吸光度（OD），样本中的人CA125/MUC16浓度与OD成正比，通过绘制标准曲线和四参数拟合软件便可计算出样本中人CA125/MUC16的浓度。
Background	The CA125 antigen，also known as MUC16，is a mucin protein that may be found in type I transmembrane or secreted forms that are used monitor the progress of epithelial ovarian cancer therapy. Expression of isoforms，proteolytic cleavage，and heavy N- and O- linked glycosylation produce forms of human CA125 that can vary from 200-5000 kDa in size. It inhibits NK cell activity，which is thought to contribute to immune evasion in peritoneal cancer and pregnancy
Unit	Kit
Specification	48T 96T