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My CartApplication：ELISA
Reactivity：Human
Sample Type：Serum，Plasma，Cell Culture Supernatant
Sensitivity：62.5pg/mL
Storage：2-8℃，6 months
Gene ID：1508
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| Name | Human CTSB ELISA Kit |
| Storage | 2-8℃，6 months |
| Detection Target | CTSB |
| Sample Type | Serum，Plasma，Cell Culture Supernatant |
| Reactivity | Human |
| Application | ELISA |
| Reaction Type | Sandwich-ELISA |
| Quantitative/Qualitative | Quantitative |
| Detection Range | 125-8000pg/mL |
| Sensitivity | 62.5pg/mL |
| Precision | Both intra-plate and inter-plate variation coefficients were less than 10% |
| Recovery | 80%-120% |
| Assay Time | 3h15min |
| Swiss Prot | P07858 |
| Gene ID | 1508 |
| Detection Principle | Solarbio ELISA试剂盒采用基于双抗体夹心法的酶联免疫吸附检测技术。将抗人CTSB(Cathepsin B)单克隆抗体包被在酶标板上；分别加入梯度稀释的标准品和预稀释的样本，标准品和样本中的人CTSB(Cathepsin B)会与酶标板上的包被抗体充分结合；洗板后加入生物素化抗人CTSB(Cathepsin B)抗体，该抗体会与板子上包被抗体捕获的标准品和样本中的人CTSB(Cathepsin B)发生特异性结合；洗板后加入辣根过氧化物酶（HRP）标记的链霉亲和素，生物素与链霉亲和素会发生高强度的非共价结合；洗板后加入显色剂底物TMB，若反应孔中样品存在不同浓度的人CTSB(Cathepsin B)，则HRP会使无色TMB变成不同深浅（正相关）的蓝色物质，加入终止液后反应孔会变成黄色；最后，在λmax=450 nm（OD=450 nm）处测定反应孔样品吸光度（OD），样本中的人CTSB(Cathepsin B)浓度与OD成正比，通过绘制标准曲线和四参数拟合软件便可计算出样本中人CTSB(Cathepsin B)的浓度。 |
| Background | Cathepsin B is the first described member of the family of lysosomal cysteine proteases. Cathepsin B possesses both endopeptidase and exopeptidase activities，in the latter case acting as a peptidyl-dipeptidase. It is known to process a number of proteins，including pro and active caspases，prorenin and SLPI. Cathepsin B is synthesized as a preproenzyme. Following removal of the signal peptide，the inactive proenzyme undergoes further modifications including removal of the pro region to result in the active enzyme. |
| Unit | Kit |
| Specification | 48T 96T |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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Cathepsin B-activatable Cyclic Antisense Oligonucleotides for Cell-specific Target Gene Knockdown In Vitro and In Vivo
Click to check >>Author：Zhongyu Wang, Xinli Fan, Guanqun Mu, Xiaoran Zhao, Qian Wang, Jing Wang, Xinjing Tang
IF：8.8000
Publish_to：Molecular Therapy-Nucleic Acids
PMID：37588686
