Human CTSB ELISA Kit_Human_ELISA_Solarbio-Global Leader in Antibody and Elisa Kit

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SKU	Tongzhou Beijing	Haidian Beijing	Wuhan	Guangzhou
SEKH-0534-48T	Inquiry	Inquiry	Inquiry	Inquiry
SEKH-0534-96T	Inquiry	Inquiry	Inquiry	Inquiry

Chinese Name	人组织蛋白酶B检测试剂盒检测试剂盒
English Name	Human CTSB ELISA Kit
Synonyms	APP secretase; APPS; Cathepsin B; Cathepsin B1; CPSBamyloid precursor protein secretase; CTSB; cysteine protease
Storage	2-8℃，6 months
Detection Target	CTSB
Sample Type	Serum，Plasma，Cell Culture Supernatant
Reactivity	Human
Application	ELISA
Reaction Type	Sandwich-ELISA
Quantitative/Qualitative	Quantitative
Detection Range	125-8000pg/mL
Sensitivity	62.5pg/mL
Precision	Both intra-plate and inter-plate variation coefficients were less than 10%
Recovery	80%-120%
Assay Time	3h15min
Swiss Prot	P07858
Gene ID	1508
Detection Principle	Solarbio ELISA试剂盒采用基于双抗体夹心法的酶联免疫吸附检测技术。将抗人CTSB(Cathepsin B)单克隆抗体包被在酶标板上；分别加入梯度稀释的标准品和预稀释的样本，标准品和样本中的人CTSB(Cathepsin B)会与酶标板上的包被抗体充分结合；洗板后加入生物素化抗人CTSB(Cathepsin B)抗体，该抗体会与板子上包被抗体捕获的标准品和样本中的人CTSB(Cathepsin B)发生特异性结合；洗板后加入辣根过氧化物酶（HRP）标记的链霉亲和素，生物素与链霉亲和素会发生高强度的非共价结合；洗板后加入显色剂底物TMB，若反应孔中样品存在不同浓度的人CTSB(Cathepsin B)，则HRP会使无色TMB变成不同深浅（正相关）的蓝色物质，加入终止液后反应孔会变成黄色；最后，在λmax=450 nm（OD=450 nm）处测定反应孔样品吸光度（OD），样本中的人CTSB(Cathepsin B)浓度与OD成正比，通过绘制标准曲线和四参数拟合软件便可计算出样本中人CTSB(Cathepsin B)的浓度。
Background	Cathepsin B is the first described member of the family of lysosomal cysteine proteases. Cathepsin B possesses both endopeptidase and exopeptidase activities，in the latter case acting as a peptidyl-dipeptidase. It is known to process a number of proteins，including pro and active caspases，prorenin and SLPI. Cathepsin B is synthesized as a preproenzyme. Following removal of the signal peptide，the inactive proenzyme undergoes further modifications including removal of the pro region to result in the active enzyme.
Unit	Kit
Specification	48T 96T