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Determination of Significance：
Superoxide dismutase (SOD, EC 1.15.1.1) is a kind of metalloenzyme widely found in organism. It is an important oxygen radical scavenger and can catalytic disproportionation of superoxide anion to form H2O2 and O2. SOD is not only the superoxide anion scavenging enzyme, but also the main H2O2 producing enzyme, which plays an important role in the biological antioxidant system.
Measurement Principle：
Superoxide anion (O2-) is produced by the xanthine and xanthine oxidase reaction system. O2- can reduce blue tetrazole to form blue formazan, which has absorbance in 560 nm. SOD can remove O2- and inhibit the formation of methionine. The darker the blue color of the reaction solution, the lower the SOD activity. The lighter the blue color of the reaction solution, the higher the activity of SOD.
Self Provided：
Spectrophotometer/microplate reader, table centrifuge, transferpettor, micro glass cuvette/96 well flat-bottom plate, cell ultrasonic crusher, mortar/ homogenizer, ice and distilled water.
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