His Label Purified Resin (TED)
Cat.No：P2011 Solarbio
Storage：2-8℃，1 year
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Allophycocyanin(APC)-Conjugated
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PI3K/Akt/mTOR
Endocrinology & Hormones
Protein Tyrosine Kinase/PTK
Angiogenesis
HIF
Immunology & Inflammation
Stem Cells
Apoptosis
Metabolic Enzyme & Protease
Autophagy
ER Stress
Cell Cycle
Cytoskeleton
DNA Damage & DNA Repai
Epigenetics
Ubiquitin
JAK/STAT
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Pipette & Dispenser & Pipette Dispensing Instrument
Oscillating Mixing & Stirring & Dispersing Instrument
Temperature Control & Sampling & Grinding Crushing Instrument
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Spectrophotometer
Centrifuge
Shaker
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Product Information
| Storage | 2-8℃，1 year |
| Unit | Bottle |
| Specification | 5ml 10ml 25ml |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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试剂盒是否能用于原核表达系统的HIS蛋白纯化吗？ 2022-08-02 08:44:47
Administrator您好，原核表达的His标签蛋白纯化也是可以的，TED螯合剂NTA形成的填料，由于其螯合方式不同，结合蛋白能力较6FF及4FF弱，但特异性和耐受性更强，样品需要低流速反复多次上柱，如果是原核表达可以选用P2010这种载量更高的。这一款更多用于真核表达蛋白纯化，能耐受更高的还原剂、螯合剂，真核分泌表达的 His 标签蛋白无需前处理即可上样，最大限度的保护蛋白活性。