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试剂盒是否能用于原核表达系统的HIS蛋白纯化吗?2022-08-02 08:44:47
Administrator您好,原核表达的His标签蛋白纯化也是可以的,TED螯合剂NTA形成的填料,由于其螯合方式不同,结合蛋白能力较6FF及4FF弱,但特异性和耐受性更强,样品需要低流速反复多次上柱,如果是原核表达可以选用P2010这种载量更高的。这一款更多用于真核表达蛋白纯化,能耐受更高的还原剂、螯合剂,真核分泌表达的 His 标签蛋白无需前处理即可上样,最大限度的保护蛋白活性。