Ascorbate Peroxidase(APX) Activity Assay Kit
Cat.No:BC0225 Solarbio
Detection Method:Spectrophotometer/Microplate Reader
Storage:Store at 2-8℃,6 months
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Ascorbate Peroxidase(APX) Activity Assay KitDetection Method:Spectrophotometer/Microplate Reader
Storage:Store at 2-8℃,6 months
Qty:
Size:
Name | Ascorbate Peroxidase(APX) Activity Assay Kit |
Detection Method | Spectrophotometer/Microplate Reader |
Storage | Store at 2-8℃,6 months |
Recommend | If 96-well plate is used for determination, 96-well UV plate should be used, recommended item numberYA0602 |
Unit | Kit |
Specification | 100T/96S |
Determination of Significance:
Ascorbate Peroxidase (APX) is an important antioxidase of plant scavenging reactive oxygen, also is one key enzyme of ascorbic acid metabolism. APX has a variety of isozymes located in chloroplast, cytoplasm, mitochondria, peroxides and glyoxylate, peroxisome and thylakoid membrane respectively. APX is the main consumer of plant AsA, which catalyzes the oxidation of AsA by H2O2. The activity of APX directly affects the content of ASA, and there is a negative correlation between APX and ASA.
Measurement Principle:
APX catalyzes the oxidation of ASA by H2O2. In this kit, the activity of APX is calculate by the oxidize rate of AsA.
Self Provided:
Refrigerated centrifuge, spectrophotometer/microplate reader, micro quartz cuvette/96 well UV flat-bottom plate, transferpettor, mortar/ homogenizer, ice and distilled water.
Note:Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.
Remark:These protocols are for reference only. Solarbio does not independently validate these methods.
Note:
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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