Reducing Sugar Content Assay Kit
Cat.No:BC0235 Solarbio
Detection Method:Spectrophotometer/Microplate Reader
Storage:Store at 2-8℃,6 months
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Reducing Sugar Content Assay KitDetection Method:Spectrophotometer/Microplate Reader
Storage:Store at 2-8℃,6 months
Qty:
Size:
Name | Reducing Sugar Content Assay Kit |
Detection Method | Spectrophotometer/Microplate Reader |
Storage | Store at 2-8℃,6 months |
Unit | Kit |
Specification | 100T/48S |
Determination of Significance:
Reducing sugar is widely found in animals, plants, microorganisms and cultured cells. Reducing sugars in plants mainly include glucose, fructose and maltose, which are the most common monosaccharides and disaccharides. Glucose and fructose are not only the main substrates of respiration, but also the substrates for further synthesis of sucrose, starch and cellulose.
Measurement Principle:
Heating can promote the formation of brownish red amino compound from 3,5-Dinitrosalicylic acid solution and reducing sugar in alkaline solution. This brownish red amino compound has a characteristic absorption peak at 540 nm. Within a certain concentration range, the content of reducing sugar has a linear relationship with the absorbance at 540 nm. According to the standard curve, the amount of reducing sugar in the sample can be calculated.
Self Provided:
Spectrophotometer/microplate reader, water-bath, table centrifuge, sonicator transferpettor, micro glass cuvette/96 well flat-bottom plate, mortar and distilled water.
Note:Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.
Remark:These protocols are for reference only. Solarbio does not independently validate these methods.
Note:
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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