ATP Content Assay Kit
Cat.No：BC0305 Solarbio
Detection Method：Spectrophotometer/Microplate Reader
Storage：Store at -20℃，6 months
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Oscillating Mixing & Stirring & Dispersing Instrument
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> Biochemical Assay Kits > Energy Metabolism Series > ATP Series > ATP Content Assay Kit
Product Information
| Reagents Required But Not Provided | Please check the manual first, there are some reagents you should prepare by yourself. |
| Name | ATP Content Assay Kit |
| Detection Method | Spectrophotometer/Microplate Reader |
| Storage | Store at -20℃，6 months |
| Recommend | If 96-well plate is used for determination, 96-well UV plate should be used, recommended item numberYA0602 |
| Unit | Kit |
| Specification | 100T/96S |
Determination of Significance：
ATP (adenosine 5'-triphosphate) is found broadly in animals, plants, microorganisms and cultured cells, which is described as the energy currency in all living systems. Detecting the content of ATP and calculating the level of energy charge can reflect the state of energy metabolism.
Measurement Principle：
Hexokinase (HK) catalyzes the synthesis of glucose and ATP into 6-phosphate glucose. 6-phosphate glucose dehydrogenase further catalyzes the dehydrogenation of glucose 6-phosphate and NADP to form NADPH. NADPH has a characteristic absorption peak at 340 nm, and the absorbance ratio of NADPH is in direct proportion to contents of ATP.
Self Provided：
Spectrophotometer/microplate reader, water bath/constant temperature incubator, refrigerated centrifuge, transferpettor, micro quartz cuvette/96 well UV flat-bottom plate, ice, mortar/ homogenizer, distilled water and chloroform.
Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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这个试剂盒的灵敏度多少啊？ 2023-05-18 11:38:41
Administrator您好，最低检出限：0.0026 μmol/mL；线性范围：0.019-3 μmol/mL