CoenzymeⅠNAD(H) Content Assay Kit

Determination of Significance：

Coenzyme I include both reduced and oxidized forms and plays a role in hydrogen transfer in biological oxidation. Oxidized coenzyme I also called nicotinamide adenine dinucleotide (NAD+) which is the coenzyme of dehydrogenase. It is an important role in glycolysis, gluconeogenesis, tricarboxylic acid cycle and respiratory chain. Intermediate product will transfer hydrogen to NAD make it become NADH(reduced coenzyme I). NADH acts as a carrier for hydrogen and synthesizes ATP by chemosmosis coupling in the respiratory chain. NADH has important physiological significance in the body. It is closely related to substance metabolism, energy metabolism, anti-cell aging, anti-oxidation and the occurrence of some diseases. A decrease in coenzyme I levels in the body can lead to cell damage or death.

Measurement Principle：

Extract the sample of NAD+ and NADH with acidic and alkaline extract solution respectively, NADH reduces the oxidized Thiazolyl Blue Tetrazolium Blue (MTT) to form formazan by hydrogen transfer from PMS, formazan has characteristic absorption at 570nm. NAD could be reduced to NADH by alcohol dehydrogenase. Further, MTT reduction method was used to detect NAD+.

Self Provided：

Microplate reader/Spectrophotometer, desk centrifuge, desk centrifuge, transferpettor, micro glass cuvette/ 96 well flat-bottom plate, mortar/ homogenizer, ice and distilled water.

Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.

Remark：These protocols are for reference only. Solarbio does not independently validate these methods.

Note：

1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.

2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.

3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.