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Determination of Significance:
Lipoxygenase (LOX) is widely found in plant tissues, especially soybean seeds. LOX catalyzes the oxidation of unsaturated fatty acids, resulting in membrane lipid peroxidation. It plays an important role in plant growth and development, maturation and aging.
Measurement Principle:
LOX catalyzes the oxidation of linoleic acid, the oxidation product has a characteristic absorption peak at 234 nm. The rate of increase in absorbance at 234 nm is measured to calculate the LOX activity.
Self Provided:
Spectrophotometer/microplate reader, micro quartz cuvette/ 96-well flat-bottom plate (UV), refrigerated centrifuge, adjustable pipette, mortar/ homogenizer, ice and distilled water
Note:Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.
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