Determination of Significance：

Glycogen is a high molecular polysaccharide composed of glucose units. It is one of the main storage forms of sugar. It is mainly stored in the liver and muscle as backup energy, and is called liver glycogen and muscle glycogen, respectively. Glycogen can regulate blood glucose concentration. Glycogen can be synthesized in the liver when blood glucose rises. When blood sugar decreases, liver glycogen is broken down into glucose to supplement blood sugar. Therefore, liver glycogen is important to maintain the relative balance of blood sugar. Muscle glycogen is a form of glycogen storage in muscles. When lots of blood sugar is consumed during strenuous exercise, muscle glycogen cannot be broken down directly into blood sugar. It must first be broken down to produce lactic acid, which is circulated to the liver with the blood, and transformed into liver glycogen through glycogen glucose.

Measurement Principle：

Determination principle: anthrone method. Glycogen is extracted with strong alkaline extract, and the glycogen content is measured using an anthrone method under strongly acidic conditions.

Self Provided：

Spectrophotometer/microplate reader, water bath,desk centrifuge, transferpettor, micro glass cuvette/96-well plate, concentrated sulfuric acid (H2SO4) and distilled water.

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