Determination of Significance:
β-1,3-GA (EC 3.2.1.73) mainly exists in plants and catalyzes the hydrolysis of β-1, 3-glucoside bond. A large number of β-1,3-GA can be induced by plant infection or other adverse conditions. Therefore, β-1,3-GA activity assay has been widely used in plant pathology and stress physiology studies.
Measurement Principle:
β-1,3-GA hydrolyzes laminarin and inner cuts β-1, 3-glucoside bond to produce reducing terminus. The enzyme activity is calculated by measuring the rate of reducing sugar production.
Self Provided:
Spectrophotometer/microplate reader, desk centrifuge, Water bath / constant temperature incubator ,adjustable pipette, micro glass cuvette/96 well flat-bottom plate, mortar/homogenizer, sonicator ,ice and distilled water.
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