Determination of Significance:
PDH widely exist in animals, plants, microorganism and cultured cells, which is the rate-limiting enzyme of acetylformic acid oxidative and decarboxylate catalyzed by Pyruvate dehydrogenase complex (PDHC). The decarboxylation of acetylformic acid forms hydroxyethyl-TPP, links glycolysis to the three carboxylic acid cycle.
Measurement Principle:
PDH catalyzes the dehydrogenation of acetylformic acid and reduct 2, 6-dichlorophenol indophenol (2,6-DCPIP), which makes the absorption of 605 nm decrease.
Self Provided:
Spectrophotometer/microplate reader, water bath, desk centrifuge, adjustable pipette, micro glass cuvette/96 well flat-bottom plate, mortar/homogenizer, ice and distilled water.
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