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Determination of Significance：
Acetyl-CoA carboxylase (ACC) catalyzes the carboxylation of acetyl CoA to malonyl CoA in vivo and is a key enzyme in the synthesis of fatty acids and many secondary metabolites. The activity of ACC determines the synthesis speed and oil content of fatty acids to a certain extent.
Measurement Principle：
ACC can catalyze acetyl coenzyme A, NaHCO3 and ATP to generate malonyl CoA, ADP and inorganic phosphorus. Molybdenum blue and phosphate generate a substance with a characteristic absorption peak at 660nm. ACC activity is determined by measuring the increase of inorganic phosphorus by ammonium molybdate phosphorus determination method.
Self Provided：
Mortar/Homogenizer/Cell Ultrasonic Crusher, Ice, Low Temperature Centrifuge, Spectrophotometer/Microplate Reader, Micro Quartz Cuvette/96 Well Flat-bottom Plate (UV plate), Water Bath; Adjustable Pipette and Distilled Water.
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