Determination of Significance：

Sucrose Phosphorylase (SP) (EC2.4.1.7) mainly exists in microorganisms and plants. Sucrose phosphorylase cleaves the glucosidic bond and catalyzes the transfer of the glucosyl group to fructose, xylose, galactose and rhamnose to synthesize the corresponding glucosyl oligosaccharides. In addition, sucrose phosphorylase can also catalyze the synthesis of arbutin from hydroquinone, which has a strong whitening effect and has important applications in the cosmetics industry.

Measurement Principle：

Sucrose phosphorylase can use phosphoric acid as the receptor to catalyze the production of glucose 1-phosphate from sucrose, which is transformed into glucose 6-phosphate under the catalysis of glucose phosphate mutase, and reduces NADP+ to NADPH under the action of glucose 6-phosphate dehydrogenase. This results in an increase in light absorption at 340nm. The sucrose phosphorylase activity can be calculated by measuring the increase rate of absorbance at 340nm.

Self Provided：

Ultraviolet spectrophotometer/microplate reader, low temperature centrifuge, constant temperature incubator, adjustable pipette, mortar/homogenizer, micro quartz cuvette/96 well UV flat -bottom plate, ice and distilled water.

Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.