Determination of Significance:
Soil phosphatase is an enzyme which catalyzes soil organic phosphate mineralization, the activity influences the decomposition and transformation of organic phosphate and its bio-availability directly, which is the indicator of evaluating the direction and intensity of soil phosphorus bio-transformation. Soil phosphatase is influenced by the content of carbon, nitrogen, available phosphorus in the soil and pH. Soil phosphatase is divided into three types: acidic, neutral and alkaline phosphatase according to the optimum pH.
Measurement Principle:
In neutral condition, soil neutral phosphatase (S-NP) can catalyzes the hydrolysis of disodium phenyl phosphate to produce phenol and disodium hydrogen phosphate, the activity of S-NP can be calculate by detecting the content of phenol.
Self Provided:
Spectrophotometer/microplate reader, micro cuvette/96 well flat-bottom plate, 37℃ constant temperature incubator, desk centrifuge, adjustable pipette, analytical balance, toluene, alcohol, ice and distilled water.
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