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Determination of Significance：
Fluorescein diacetate (FDA) hydrolysis reaction is one of the most important biological indicators in the study of soil quality, which can reflect the activity of soil microbial, the change of soil quality and the transformation of organic matter in ecosystem.
Measurement Principle：
FDA is a colorless compound, which can be hydrolyzed by many soil enzymes in the medium. After dehydration reaction, fluorescein is the final product of enzymatic hydrolysis. The fluorescein is stable and not easy to be decomposed, and has a strong absorption peak at 490 nm. The activity of FDA hydrolase can be calculated by detecting the change of absorption value at 490 nm.
Self Provided：
Balance, tabletop centrifuge, spectrophotometer/microplate reader, micro glass cuvette/96 well flat-bottom plate, constant temperature water bath, acetone, 30-50 mesh sieve (or smaller).
Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.
