Lysosomes are bubble-like structures surrounded by a unit membrane containing a variety of acid hydrolases. Lysosomes contain more than 40 hydrolytic enzymes, including proteases, nucleic acid degrading enzymes and glycosidases. Its main function is the digestion of intracellular substances. This exolysosome is closely related to organ formation, the regulation of hormone secretion, and the occurrence of certain diseases. Lysosomal protein extraction Kit provides a complete set of reagents for lysosomal protein extraction from a variety of animal cell and solid soft and hard tissue samples. It is suitable for extracting total lysosomal protein from various primary or pass-through cells and various solid tissues, such as brain, spinal cord, nerve junction or fiber, fat, liver, digestive tract, kidney, heart, muscle, blood vessel, connective tissue and other animal tissues. This kit contains a unique formulation that effectively dissolves membrane components. The kit contains a protease inhibitor mixture that prevents protease from degrading the protein and provides a guarantee for extracting high purity protein. The protein extracted from this kit can be used for downstream protein research experiments such as Western Blotting, protein electrophoresis, immunoprecipitation, ELISA, transcriptional activity analysis, Gel shift gel blocking experiment, enzyme activity determination, etc. The protein extracted by this kit is an active protein with natural protein conformation.

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