The endoplasmic reticulum is present in all kinds of eukaryotic cells except mature red blood cells of mammals. The endoplasmic reticulum (ER) is a system of interconnecting lamellar gaps or small tubules composed of biofilms. The space between the membranes is called the cisterna, which is usually not directly connected with the extracellular Spaces and the cytoplasmic matrix. On the one hand, this membranous duct system constitutes the transport pathway of intracellular substances, on the other hand, it provides a broad reaction area for various enzyme reactions in the cell. The functions of the endoplasmic reticulum are related to the synthesis of proteins, the synthesis of sugars and lipids, detoxification and assimilation, and also have the function of transporting proteins. The ER protein extraction kit can be used to extract ER protein from various animal cells and solid soft and hard tissue samples. The protein extracted from this kit can be used for downstream protein research experiments such as Western Blotting, protein electrophoresis, immunoprecipitation, ELISA, transcriptional activity analysis, Gel shift gel blocking experiment, enzyme activity determination, etc. The protein extracted by this kit is an active protein with natural protein conformation. This kit does not contain EDTA and is compatible with downstream applications such as metal chelation and chromatography.

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