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My Cart> Biochemical Assay Kits > Energy Metabolism Series > Mitochondrial Respiratory Chain Series > Mitochondrial complex I/NADH-CoQ reductase Activity Assay KitDetection Method：Spectrophotometer/Microplate Reader
Storage：Store at -20℃，6 months
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| Reagents Required But Not Provided | Please check the manual first, there are some reagents you should prepare by yourself. |
| Name | Mitochondrial complex I/NADH-CoQ reductase Activity Assay Kit |
| Detection Method | Spectrophotometer/Microplate Reader |
| Storage | Store at -20℃，6 months |
| Recommend | If 96-well plate is used for determination, 96-well UV plate should be used, recommended item numberYA0602 |
| Unit | Kit |
| Specification | 100T/96S |
Determination of Significance：
Mitochondrial Respiratory Chain Complex Ⅰ (EC 1.6.5.3) same as NADH-CoQ reductase or NADH dehydrogenase, exists widely in mitochondria of animals, plants, microorganisms and cultured cells, which is the largest protein complex in inner mitochondrial membrane. This enzyme catalyzes a pair of electrons transfer from NADH to CoQ, reduce O2 to O2-, which is the main site of O2- production on the respiratory electron transport chain. The enzyme activity of Complex Ⅰ reflects the state of respiratory electron transport chain (ETC) and produce state of ROS.
Measurement Principle：
Complex Ⅰ catalyzes NADH to form NAD+, the activity of this enzyme can be calculated by detecting the oxidation rate of NADH at 340 nm.
Self Provided：
Ultraviolet spectrophotometer/microplate reader, micro quartz cuvette/96 well flat-bottom UV plate, water bath/constant temperature incubator, desk centrifuge, adjustable pipette, mortar/homogenizer/cell ultrasonic crusher, acetone, ice and distilled water.
Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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