Mitochondrial protein Extraction Kit provides a complete set of reagents for the extraction of mitochondrial proteins from a variety of primary or successive cells and a variety of solid tissues such as brain, spinal cord, nerve junctions or fibers, fat, liver, digestive tract, kidney, heart, muscle, blood vessels, connective tissue and other mammalian tissues. The extraction process is simple and convenient. The prepared mitochondrial proteins not only have high purity and natural activity, but also have little cross-contamination. This kit contains a unique formulation that effectively dissolves mitochondrial membrane components. The kit contains a protease inhibitor mixture that prevents protease from degrading the protein and provides a guarantee for extracting high purity proteins. The protein extracted from this kit can be used for downstream protein research experiments such as Western Blotting, protein electrophoresis, immunoprecipitation, ELISA, transcriptional activity analysis, Gel shift gel blocking experiment, enzyme activity determination, etc. The protein extracted by this kit is an active protein with natural protein conformation. This kit does not contain EDTA and is compatible with downstream applications such as metal chelation and chromatography.
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线粒体提取结束之后,该如何判断线粒体的浓度或者是纯度?2022-08-25 20:12:08
Administrator可以用线粒体染色试剂(健那绿)染色观察