Whole Blood Total Protein extraction kit is available for the extraction of total protein from a variety of animal anticoagulant/non-anticoagulant whole blood samples. This kit contains a unique formulation that effectively dissolves cell membrane components, including the plasma membrane, the nuclear membrane, and various organelle membranes. The kit contains a protease inhibitor mixture that prevents protease from degrading the protein and provides a guarantee for extracting high purity egg whites. The protein extracted from this kit can be used for downstream protein research experiments such as Western Blotting, protein electrophoresis, immunoprecipitation, ELISA, transcriptional activity analysis, Gel shift gel blocking experiment, enzyme activity determination, etc. All components of this kit do not contain detergent components, and the composition of the final protein sample has no influence on downstream applications such as NI column purification, molecular sieve, ion exchange, affinity purification, etc. The protein extraction components of this kit do not contain detergent components that cannot be removed by dialysis, and do not contain SDS, Triton X_x0002_100, chaps and other components that may affect the mass spectrometry experiment. After dialysis or desalting treatment, the final protein sample will not contain detergent, high concentration salt and other effects. Basically, it can satisfy any downstream proteomic related experimental research. The protein extracted by this kit is an active protein with natural protein conformation, which has a wide range of downstream applications. The ability of the extract to lyse cells is mild, and the lyse time needs to be optimized according to the actual sample situation. This kit does not contain EDTA and is compatible with downstream applications such as metal chelation and chromatography.

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