Pyruvate Kinase(PK) Activity Assay Kit
Cat.No：BC0545 Solarbio
Storage：Store at -20℃，6 months
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> Biochemical Assay Kits > Carbohydrate Metabolism Series > Glycolysis Series > Pyruvate Kinase(PK) Activity Assay Kit
Product Information
| Name | Pyruvate Kinase(PK) Activity Assay Kit |
| Detection Method | Spectrophotometer/Microplate Reader |
| Storage | Store at -20℃，6 months |
| Recommend | If 96-well plate is used for determination, 96-well UV plate should be used, recommended item numberYA0602 |
| Unit | Kit |
| Specification | 100T/96S |
Determination of Significance：
Pyruvate Kinase (PK, EC 2.7.1.40) is widely exists in animals, plants, microorganisms and cultured cells. It could catalyze the final step of the glycolysis process. PK is one of the major rate-limiting enzymes in the glycolysis process and one of the key enzymes for ATP production. Therefore, the determination of PK activity is of great significance.
Measurement Principle：
PK catalyzes the generation of ATP and pyruvate from phosphoenolpyruvate and ADP. Lactate dehydrogenase further catalyze NADH and pyruvate to generate lactic acid and NAD+. The NADH degradation rate can measured at 340 nm to reflect the activity of PK.
Self Provided：
Spectrophotometer/Microplate reader, table centrifuge, water-bath, adjustable pipette, micro quartz cuvette/96 well flat-bottom plate (UV plate), mortar/homogenizer, ice and distilled water.
Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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