CoenzymeⅡ NADP(H) Content Assay Kit (WST colorimetry)
Cat.No：BC5200 Solarbio
Detection Method：Spectrophotometer
Storage：Store at -20℃，6 months
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> Biochemical Assay Kits > Protein ＆ Enzyme Series > Coenzyme Ⅱ Series > CoenzymeⅡ NADP(H) Content Assay Kit (WST colorimetry)
Product Information
| Name | CoenzymeⅡ NADP(H) Content Assay Kit (WST colorimetry) |
| Detection Method | Spectrophotometer |
| Storage | Store at -20℃，6 months |
| Unit | Kit |
| Specification | 50T/24S |
Determination of Significance：
Coenzyme II NADP(H) is widely present in animals, plants, microorganisms and cultured cells. The determination of NADP+ and NADPH content can calculate the content of NADP (NADPH + NADP+) and the ratio of NADPH/NADP+, and its changes are related to the pentose phosphate pathway and biosynthesis and Antioxidative responses are closely related. The NADPH/NADP+ ratio is not only one of the main markers of cellular redox state, but also plays an important regulatory role in the PPP pathway, biosynthesis and antioxidant metabolism.
Measurement Principle：
The NADP+ and NADPH in the samples were extracted with acidic and basic extraction solutions, respectively. Under the action of 1-mPMS, WST-1 can react with NADPH to produce water-soluble formazan, which has a characteristic absorption peak at 450nm, while NADP+ can be reduced to NADPH by 6-phosphate glucose dehydrogenase, which is further detected by WST-1.
Self Provided：
Spectrophotometer, centrifuge, water bath, mortar/homogenizer, sonicator, adjustable pipette, 1 mL glass cuvette, ice, and distilled water.
Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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Related Paper
ROS-mediated TCA cycle is greatly related to the UV resistance of Bacillus thuringiensis
Click to check >>Author：Xi Du, Yile Zhang, Haonan Wu, Weilong Tan, Enjiong Huang, Xiong Guan, Lingling Zhang
IF：4.9660
Publish_to：PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
PMID：37248007
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