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Determination of Significance：
Coenzyme II NADP(H) is widely present in animals, plants, microorganisms and cultured cells. The determination of NADP+ and NADPH content can calculate the content of NADP (NADPH + NADP+) and the ratio of NADPH/NADP+, and its changes are related to the pentose phosphate pathway and biosynthesis and Antioxidative responses are closely related. The NADPH/NADP+ ratio is not only one of the main markers of cellular redox state, but also plays an important regulatory role in the PPP pathway, biosynthesis and antioxidant metabolism.
Measurement Principle：
The NADP+ and NADPH in the samples were extracted with acidic and basic extraction solutions, respectively. Under the action of 1-mPMS, WST-1 can react with NADPH to produce water-soluble formazan, which has a characteristic absorption peak at 450nm, while NADP+ can be reduced to NADPH by 6-phosphate glucose dehydrogenase, which is further detected by WST-1.
Self Provided：
Spectrophotometer, centrifuge, water bath, mortar/homogenizer, sonicator, adjustable pipette, 1 mL glass cuvette, ice, and distilled water.
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