CoenzymeⅡ NADP(H) Content Assay Kit (WST colorimetry)
Cat.No：BC5205 Solarbio
Detection Method：Spectrophotometer/Microplate Reader
Storage：Store at -20℃，6 months
Qty：
-
+
Size：
{{cart_num}}
My CartTel: +86 010 50973105
Products
Secondary Antibodies
SAlexa Fluor-Conjugated
Other Fluorescent-Conjugated
Other-Conjugated
Colloidal Gold Reagent
Alkaline Phosphatase(AP)-Conjugated
Colloidal Gold-Conjugated
FITC-Conjugated
Biotin-Conjugated
Purified Secondary Antibodies
Allophycocyanin(APC)-Conjugated
CY Fluorescent-Conjugated
Phycoglobin(PE)-Conjugated
RBTIC-Conjugated
Peroxidase (HRP)-Conjugated
Secondary Antibodies
SAlexa Fluor-Conjugated
Other Fluorescent-Conjugated
Other-Conjugated
Colloidal Gold Reagent
Alkaline Phosphatase(AP)-Conjugated
Colloidal Gold-Conjugated
FITC-Conjugated
Biotin-Conjugated
Purified Secondary Antibodies
Allophycocyanin(APC)-Conjugated
CY Fluorescent-Conjugated
Phycoglobin(PE)-Conjugated
RBTIC-Conjugated
Peroxidase (HRP)-Conjugated
Inhibitors & Antagonists & Agonists
PI3K/Akt/mTOR
Endocrinology & Hormones
Protein Tyrosine Kinase/PTK
Angiogenesis
HIF
Immunology & Inflammation
Stem Cells
Apoptosis
Metabolic Enzyme & Protease
Autophagy
ER Stress
Cell Cycle
Cytoskeleton
DNA Damage & DNA Repai
Epigenetics
Ubiquitin
JAK/STAT
GPCR & G Protein
Neuronal Signal Pathway
MAPK
NF-κB
Membrane Transporter & Ion Channel
TGF-β/Smad
Hippo
Antibody-drug Conjugate/ADC Related
Others
Inhibitors & Antagonists & Agonists
PI3K/Akt/mTOR
Endocrinology & Hormones
Protein Tyrosine Kinase/PTK
Angiogenesis
HIF
Immunology & Inflammation
Stem Cells
Apoptosis
Metabolic Enzyme & Protease
Autophagy
ER Stress
Cell Cycle
Cytoskeleton
DNA Damage & DNA Repai
Epigenetics
Ubiquitin
JAK/STAT
GPCR & G Protein
Neuronal Signal Pathway
MAPK
NF-κB
Membrane Transporter & Ion Channel
TGF-β/Smad
Hippo
Antibody-drug Conjugate/ADC Related
Others
Instruments
Pipette & Dispenser & Pipette Dispensing Instrument
Oscillating Mixing & Stirring & Dispersing Instrument
Temperature Control & Sampling & Grinding Crushing Instrument
Sterilization & Filtration Instruments
PCR Instrument & Light Glue Instrument
Spectrophotometer
Centrifuge
Shaker
Vacuum Pump
Other Small Instruments
Instruments
Pipette & Dispenser & Pipette Dispensing Instrument
Oscillating Mixing & Stirring & Dispersing Instrument
Temperature Control & Sampling & Grinding Crushing Instrument
Sterilization & Filtration Instruments
PCR Instrument & Light Glue Instrument
Spectrophotometer
Centrifuge
Shaker
Vacuum Pump
Other Small Instruments
Your Position: Home
> Biochemical Assay Kits > Protein ＆ Enzyme Series > Coenzyme Ⅱ Series > CoenzymeⅡ NADP(H) Content Assay Kit (WST colorimetry)
Product Information
| Name | CoenzymeⅡ NADP(H) Content Assay Kit (WST colorimetry) |
| Detection Method | Spectrophotometer/Microplate Reader |
| Storage | Store at -20℃，6 months |
| Unit | Kit |
| Specification | 100T/48S |
Determination of Significance：
Coenzyme II NADP(H) is widely present in animals, plants, microorganisms and cultured cells. The determination of NADP+ and NADPH content can calculate the content of NADP (NADPH + NADP+) and the ratio of NADPH/NADP+, and its changes are related to the pentose phosphate pathway and biosynthesis and Antioxidative responses are closely related. The NADPH/NADP+ ratio is not only one of the main markers of cellular redox state, but also plays an important regulatory role in the PPP pathway, biosynthesis and antioxidant metabolism.
Measurement Principle：
The NADP+ and NADPH in the samples were extracted with acidic and basic extraction solutions, respectively. Under the action of 1-mPMS, WST-1 can react with NADPH to produce water-soluble formazan, which has a characteristic absorption peak at 450nm, while NADP+ can be reduced to NADPH by 6-phosphate glucose dehydrogenase, which is further detected by WST-1.
Self Provided：
Spectrophotometer/microplate reader, centrifuge, water bath, mortar/homogenizer, sonicator, adjustable pipette, micro glass cuvettes/96 well plates, ice, and distilled water.
Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
Experimental Images
Sorry, there is no experimental images.
Related Products
Sorry, there is no more information.
Related Paper
Sorry, there is no more information.
Technical Q&A
Q&A
（Total 0
User Q&A）
- There is not question and answer now, come and send an inquiry please.
My question
Service for Scientific Research, Responsible for the Life Science
Contact Us
Address: No 85, Liandong U Valley, Middle Zone, 101102, Tongzhou Dist,
Beijing, China
Tel: +86 010 50973105
Email: info@solarbio.com
Order Guide
Service Guarantee
Resources Download
Copyright © 2005-2021 Solarbio all rights reserved.