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Determination of Significance：
L-Lactate dehydrogenase (L-LDH or LD) is the terminal enzyme of the glycolysis pathway which is found in nearly all living cells (animals, plants, and prokaryotes). L-LDH catalyzes the conversion of lactate to pyruvic acid and back, as it converts NAD+ to NADH and back.
Measurement Principle：
NAD+ and lactic acid are oxidized to pyruvic acid by the catalysis of L-LDH. Pyruvate further reacted with 2,4-dinitrophenylhydrazide to form pyruvate dinitrobenzone, which show brown red color in alkaline solution and the color depth is proportional to the concentration of pyruvate.
Self Provided：
Spectrophotometer/Microplate reader, thermostat water bath, desk centrifuge, adjustable pipette, micro glass cuvette/96 well flat-bottom plate, mortar/homogenizer, ice, distilled water.
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