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Determination of Significance：
Pyruvate carboxylase (PC, EC 6.4.1.1) is widely present in mitochondria of animals, molds and yeast, but is not found in plants and most bacteria. PC is the main postreaction for oxaloacetate, and is the first rate-limiting enzyme in the gluconeogenesis process.
Measurement Principle：
PC irreversibly catalyzes pyruvate, ATP, CO2 and water to oxaloacetate, ADP and Pi, malic dehydrogenase further catalyzes the formation of malic acid and NAD+ from acetoacetic acid and NADH. The enzyme activity of PC can be reflected by detecting the oxidation rate of NADH at 340 nm.
Self Provided：
Ultraviolet spectrophotometer/microplate reader, desk centrifuge, water bath, adjustable pipette, micro quartz cuvette/96 well flat-bottom UV plate, mortar/homogenizer, ice and distilled water.
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