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Determination of Significance：
Aldehyde dehydrogenase (EC 1.2.1.10) is a kind of aldehyde dehydrogenase. It widely exists in various animals, plants and microorganisms. In the presence of coenzyme I, it can catalyze the dehydrogenation of some primary or secondary alcohols including ethanol, aldehydes or ketones. In humans and many animals, mitochondrial acetaldehyde dehydrogenase can transform harmful alcohols. So in the study of cell detoxification, glyoxal dehydrogenase is highly concerned; Aldehyde dehydrogenase is widely used in molecular biology and detection of related diseases.
Measurement Principle：
Acetaldehyde dehydrogenase catalyzes the conversion of acetaldehyde and NAD+ to acetic acid and NADH. The activity of aldehyde dehydrogenase can be calculated by the change of absorbance value of NADH at 340 nm.
Self Provided：
Ultraviolet spectrophotometer/microplate reader, desk centrifuge, water-bath/ constant temperature incubator, adjustable pipette, micro quartz cuvette/96 well flat-bottom UV plate, mortar /homogenizer/ sonicator, ice and distilled water.
Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.
你好我有几个问题：
①说明书中的液体包括血清吗
②可以测定血清中的ALDH的活性吗
2022-08-15 21:52:55
Administrator您好，是包含血清这一类液体样本的，这类液体样本直接检测不涉及到加提取液破碎提取。