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Determination of Significance：
Chitinases are found in the shells of crustaceans such as shrimps, crabs and insects, and in the organs of mollusks (such as squid cartilage), as well as in the cell walls of fungi. Chitinase (EC 3.2.1.14) can catalyze the hydrolysis of chitin, which has the function of resisting fungal infection and become the research hotspot of antifungal diseases.
Measurement Principle：
Chitinase hydrolyzes chitin to produce N-acetylglucosamine, and the intermediate compound produced by co-heating of N-acetylglucosamine with alkali can further react with p-dimethylaminobenzaldehyde to produce a chromogenic substance, which has the characteristic absorption peaks at 585nm, and the rate of increase in absorbance reflects the activity of chitinase.
Self Provided：
Scales, water bath/constant temperature incubator, desk centrifuge, spectrophotometer/microplate reader, micro glass cuvette/96 well flat-bottom plate, mortar/homogenizer, distilled water, ultrasonic crusher.
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