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Determination of Significance：
Mammalian CarE also known as aliphatic esterase (aliesterase) is widely distributed in tissue and organism, belonging to the serine hydrolase family. CarE catalyze hydrolysis of endogenous and exogenous substances containing ester bonds, amide bonds, and thioester bonds, but can’t catalyze hydrolysis of acetylcholine and its analogues. CarE take part in lipid transport and metabolism, and related with detoxification and metabolism of many drugs, environmental poisons and carcinogens. Organophosphorus pesticides can bind to CarE and inhibit CarE activity.
Measurement Principle：
CarE can catalyze acetic acid-1-naphthalene ester to produce naphthalene ester, solid blue color development. Determination of 450 nm light absorption increase rate could calculate CarE activity.
Self Provided：
Spectrophotometer/Microplate Reader, Micro Glass Cuvette/96 Well Flat-Bottom plate, Low Temperature Centrifuge, Water-Bath, Adjustable Pipette, Distilled Water, Anhydrous Ethanol.
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