English
中文
Determination of Significance：
Glutamine synthetase (GS) mainly exists in plants, is one of the key enzymes of ammonia assimilation in organism, which can catalytic synthesis of glutamine by ammonium ion and glutamic acid. The synthesis of glutamine not only prevents excessive ammonium ions from being toxic to organisms, but glutamine is also the main storage and transport form of ammonia.
Measurement Principle：
GS catalyzes the synthesis of glutamine from ammonium and glutamic acid in the presence of ATP and Mg2+. Glutamine is further converted to gamma-glutamyl hydroxamic acid, which can form a red complex with iron under acidic condition. This complex has a maximum absorption peak at 540 nm.
Self Provided：
Spectrophotometer/Microplate Reader, Water Bath, Desk Centrifuge, Adjustable Pipette, Micro Glass Cuvette/96 Well Flat-bottom Plate, Mortar/Homogenizer/Cell Ultrasonic Crusher, Ice and Distilled Water.
Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.
