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My Cart> Biochemical Assay Kits > Energy Metabolism Series > Mitochondrial Respiratory Chain Series > Mitochondrial Complex IV / Cytochrome C Oxidase Activity Assay KitDetection Method：Spectrophotometer/Microplate Reader
Storage：Store at -20℃，6 months
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| Name | Mitochondrial Complex IV / Cytochrome C Oxidase Activity Assay Kit |
| Detection Method | Spectrophotometer/Microplate Reader |
| Storage | Store at -20℃，6 months |
| Unit | Kit |
| Specification | 100T/96S |
Determination of Significance：
Mitochondrial Respiratory Chain Complex IV also knows as cytochrome c oxidase, is a common component of the main and branch of mitochondrial respiratory electron transport chain, and finally transfer electrons to oxygen to generate water.
Measurement Principle：
Reduced cytochrome C has a characteristic absorption peak at 550 nm, mitochondrial complex Ⅳ catalyzes the formation of oxidized cytochrome C from reduced cytochrome C. The enzyme activity of Complex IV can be calculated by detecting the decrease rate of reduced cytochrome C at 550 nm.
Self Provided：
Spectrophotometer/Microplate Reader, water bath/constant temperature incubator, desk centrifuge, adjustable pipette, micro glass cuvette/96 well flat-bottom plate, mortar/homogenizer/cell ultrasonic crusher, ice and distilled water.
Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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