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Determination of Significance：
Succinate Dehydrogenase (SDH, EC 1.3.5.1) is widely found in animals, plants, microorganisms and cultured cells. SDH is a marker enzyme of mitochondria, which is a membrane binding enzyme located in the inner membrane of mitochondria. It is also one of the key points of respiratory electron transfer and oxidative phosphorylation. In addition, it provides electrons for the respiratory chain of various prokaryotic cells.
Measurement Principle：
SDH can catalyzes the dehydrogenation of succinic acid to fumaric acid. The dehydrogenation can reduce 2,6-dichlorophenol indophenol (DCPIP) under the transfer of phenazine dimethyl sulfate (PMS). 2,6-DCPIP has a characteristic absorption peak at 600 nm. The reduction rate of 2,6-DCPIP is determined by the change of absorbance at 600 nm, which represents the activity of SDH enzyme.
Self Provided：
Spectrophotometer/Microplate reader, water-bath, tabletop centrifuge, adjustable pipette, micro cuvette/96 well flat-bottom plate, mortar/homogenizer, ice and distilled water.
Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.
500万个细菌怎么定量啊
2022-07-15 09:37:50
Administrator可采用血球计数法，电子计数器计数法，细菌悬液比浊法等。具体操作方法可自行查阅相关资料及文献。