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Determination of Significance：
Pyruvate Decarboxylase (PDC) exists in yeast mainly, which is one of the key enzymes in ethanol fermentation.
Measurement Principle：
PDC catalyzes pyruvate decarboxylation to form acetaldehyde. The addition of alcohol dehydrogenase (ADH) can further catalyze the reduction of aldehydes by NADH to ethanol and NAD+. NADH has an absorbance at 340 nm but NAD+ not, the activity of PDC can be calculated by measuring decrease rate of absorption at 340 nm.
Self Provided：
Spectrophotometer/Microplate Reader, Micro Quartz Cuvette/ 96 Well Flat-bottom Plate (UV Plate), Water-bath/Constant Temperature Incubator, Desk Centrifuge, Adjustable Pipette, Mortar/Homogenizer/Cell Ultrasonic Crusher, Ice and Distilled Water.
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