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Determination of Significance：
Alcohol dehydrogenase (ADH) is a key enzyme in the metabolism of short chain alcohols. It catalyzes the reversible conversion of ethanol and acetaldehyde, and plays an important role in many physiological processes. In mammals, ADH is mainly produced in the liver. Liver damage causes ADH to be released into serum. The activity of serum ADH reflects whether the liver function is abnormal.
Measurement Principle：
ADH catalyzes the reduction of acetaldehyde by NADH to ethanol and NAD+. NADH has an absorption peak at 340 nm but NAD+ not, the activity of ADH is calculated by measuring the rate of absorbance decline at 340 nm.
Self Provided：
Mortar/Homogenizer/Cell Ultrasonic Crusher, Ice, Low Temperature Centrifuge, Spectrophotometer/Microplate Reader, Micro Quartz Cuvette/96 Well Flat-bottom Plate (UV plate), Water Bath; Adjustable Pipette and Distilled Water.
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