Xanthine Oxidase(XOD) Activity Assay Kit
Cat.No：BC1095 Solarbio
Detection Method：Spectrophotometer/Microplate Reader
Storage：Store at 2-8℃，6 months
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> Biochemical Assay Kits > REDOX Series > Oxidation Series > Xanthine Oxidase(XOD) Activity Assay Kit
Product Information
| Name | Xanthine Oxidase(XOD) Activity Assay Kit |
| Detection Method | Spectrophotometer/Microplate Reader |
| Storage | Store at 2-8℃，6 months |
| Unit | Kit |
| Specification | 100T/96S |
Determination of Significance：
XOD (EC 1.17.3.2) catalyzes the oxidation of hypoxanthine to xanthine and superoxide anion, which is one of the main sources of active oxygen and is also one of the key enzymes of nucleotide metabolism. XOD is mainly distributed in mammalian heart, lung, liver and other tissues. When liver function impaired, XOD is released into serum in a large amount, which has specific significance for the diagnosis of liver damage.
Measurement Principle：
XOD catalyzes hypoxanthine to produce xanthine and superoxide anion. Superoxide anion reacts with hydroxylamine hydrochloride to form NO2-, and the NO2- under the action of p-aminobenzenesulfonamide and naphthalene ethylenediamine hydrochloride is produced a red azo compound with a characteristic absorption peak at 530 nm. The color depth is linear with XOD activity.
Self Provided：
Spectrophotometer/Microplate reader, constant temperature foster box/water-bath, centrifuge, micro glass cuvette/96-well flat-bottom plate, adjustable transferpettor, mortar/homogenizer, ice and distilled water.
Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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Click to check >>Author：Weiqi Cui, Hui Zhou, Jingxian Zhang, Junwei Zhang, Deqiao Wu, Ying Rong, Fanglin Liu, Junhui Liu, Haiyan Liu, Bo Wei, Youcai Tang, Xinglin Liao, Xia Xu
IF：4.5000
Publish_to：ENVIRONMENTAL TOXICOLOGY
PMID：37347548
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请问微量法和分光光度法哪个更准确？还有elisa法呢 2022-10-06 21:51:25
Administrator您好，分光法和微量法原理相同，设计的不同大小的反应体系，分别适配比色皿和酶标板检测的，分光法更大的反应体系检测读数稳定性受误差影响要更小，微量法优势在于酶标仪能快速对多个样本完成检测。ELISA方法检测从原理上说检测灵敏度要比生化法灵敏度高，但会受到种属来源限定，生化法是不限制种属来源的，能否检测到取决于物质含量活性。
请问你们家的黄嘌呤氧化还原酶试剂盒，测溶血的血清会有影响吗 2022-07-18 10:48:25
Administrator该试剂盒反应生成物显示紫红色，在530nm处检测其吸收峰。溶血的血清因血红蛋白颜色会影响最终吸光值检测，导致数据不准确。不建议使用。