English
中文
Determination of Significance：
MEwidely exist in microbe, culturing cells, animal and plant cytoplasm. Especially it has high activity in plant tissue. ME catalyzes the reversible reaction of malic acid oxidative decarboxylation to produce pyruvic acid and CO2 with the reduction of NAD(P)+, which is the crucial enzyme of malic acid metabolism. The activity of ME is related with biosynthesis and anti-oxidation. In recent years, the determination of plant ME activity become a hot spot in antioxidant research. According the coenzyme specificity and substrate specificity, ME is divided into NAD-ME (EC1.1.1.38) and NADP-ME (EC1.1.1.40).
Measurement Principle：
NADP-ME catalyzes the reduction of NADP+ to NADPH, detect the increase rate of NADPH at 340 nm.
Self Provided：
Spectrophotometer/Microplate reader, low temperature centrifuge, water-bath, adjustable pipette, micro quartz cuvette/96 well flat-bottom plate(UV plate), mortar/homogenizer and distilled water.
Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.
