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Determination of Significance：
MEwidely exists in microbe, culturing cells, animal and plantcytoplasm. Especially it has high activity in plant tissue. ME catalyzesthe reversible reaction of malic acidoxidative decarboxylation to produce pyruvic acid and CO2with the reduction of NAD(P)+, which is the crucial enzyme of malic acid metabolism. The activity of ME is related with biosynthesis and anti-oxidation. In recent years, the determination of plant ME activity become a hot spot in antioxidant research. According the coenzyme specificity and substrate specificity, ME is divided into NAD-ME(EC1.1.1.38) and NADP-ME(EC1.1.1.40).
Measurement Principle：
NADP-ME catalyzesthe reduction of NADP+ to NADPH,detect the increase rate of NADPH at 340nm.
Self Provided：
Spectrophotometer/microplate reader, low temperature centrifuge, water bath, adjustable pipette, micro quartz cuvette/96 well flat-bottom plate(UV plate), mortar/homogenizer and distilled water.
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