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Determination of Significance：
Creatine kinase (CK) (EC 2.7.3.2) is also known as creatine phosphokinase, which mainly exists in heart, muscle and brain. It can reversibly catalyze the trans-phosphoryl reaction between creatine and ATP. It is an important kinase directly related to cell energy transport, muscle contraction and ATP regeneration.
Measurement Principle：
CK catalyzes creatine phosphate and ADP to generate creatine and ATP, hexokinase catalyzes ATP and glucose to generate glucose-6-phosphate, and glucose-6-phosphate dehydrogenase catalyzes glucose-6-phosphate and NADP+ to generate NADPH, resulting in an increase of 340 nm light absorption value, which is used to express CK enzyme activity.
Self Provided：
Scales, low temperature centrifuge, constant temperature water bath, spectrophotometer/microplate reader, micro quartz cuvette/96 wellflat-bottom(UV) plate and distilled water.
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