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My Cart> Antibodies > Loading Control Antibodies > Plasmalemma > Anti-TFRC Polyclonal AntibodyReactivity：Human
Application：IF,WB,IHC,ELISA
Clone Type：Polyclonal Antibody
Storage：Store at -20℃. Avoid freeze/thaw cycles.
Gene ID：7037
Host：Rabbit
Qty：
Size：
| Name | Anti-TFRC Polyclonal Antibody |
| Host | Rabbit |
| Clone Type | Polyclonal Antibody |
| Subtype | IgG |
| Predicted Reactivity | Orangutan;Horse;Cat;Dog;Pig;Chicken |
| Dilution Ratio | WB 1:1000-3000. IHC 1:100-300. IF 1:50-200. |
| Target | TFRC |
| Background | Cellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied transferrin receptor into specialized endosomes (PubMed:26214738).Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system (By similarity).A second ligand, the heditary hemochromatosis protein HFE, competes for binding with transferrin for an overlapping C-terminal binding site. Positively regulates T and B cell proliferation through iron uptake (PubMed:26642240).Acts as a lipid sensor that regulates mitochondrial fusion by regulating activation of the JNK pathway (PubMed:26214738). |
| Swiss Prot | P02786 |
| Gene ID | 7037 |
| Subcellular Location | Cell membrane Secreted |
| Immunogen | Recombinant protein of human TFRC |
| Public Immunogen Range | 101-450aa |
| Calculated MW | 85kDa |
| Purification Method | Affinity purification |
| Storage Buffer | PBS with 50% Glycerol，0.03% Proclin300，0.5% BSA，pH 7.3. |
| Storage | Store at -20℃. Avoid freeze/thaw cycles. |
| Unit | Piece |
| Y-Dilution Ratio | WB 1:1000-3000. IHC 1:100-300. IF 1:50-200. ELISA 1:8000-80000. |
| Specification | 50ul 100ul |
| Reactivity | Human |
| Application | IF WB IHC ELISA |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Kindly reminder：Quick spin before use. If you have any questions, please contact us.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
Western blot analysis with TFRC antibody diluted at 1:2000;Lane: 293T,Jurkat,HEK293.
Immunohistochemistry of paraffin-embedded Human colorectal cancer with TFRC antibody diluted at 1:200.
Immunofluorescence analysis of HeLa cells using TFRC antibody diluted at 1:100.
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