Reduced Glutathione(GSH) Content Assay Kit
Cat.No:BC1175 Solarbio
Detection Method:Spectrophotometer/Microplate Reader
Storage:Store at 2-8℃,6 months
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Reduced Glutathione(GSH) Content Assay KitDetection Method:Spectrophotometer/Microplate Reader
Storage:Store at 2-8℃,6 months
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Name | Reduced Glutathione(GSH) Content Assay Kit |
Detection Method | Spectrophotometer/Microplate Reader |
Storage | Store at 2-8℃,6 months |
Unit | Kit |
Specification | 100T/96S |
Determination of Significance:
Glutathione is a natural tripeptide composed of glutamic acid (Glu), cysteine (Cys) and glycine (Gly). It is a kind of compound containing sulfhydryl group (-SH), which widely exists in animal tissue, plant tissue, microorganism and yeast.
Measurement Principle:
Glutathione can react with 5,5'-dithiobis-(2-nitrobenzoic acid) (DTNB) to produce 2-nitro-5-mercaptobenzoic acid and glutathione disulfide (GSSG). 2-nitro-5-mercaptobenzoic acid is a yellow product with the maximum absorption at 412 nm.
Self Provided:
Analytical balance, mortar/homogenizer, low temperature centrifuge, water bath, adjustable pipette, spectrophotometer/microplate reader, micro glass cuvette or 96 well flat-bottom plate and distilled water.
Note:Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.
Remark:These protocols are for reference only. Solarbio does not independently validate these methods.
Note:
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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您好,说明书中加样那一步,标准溶液的是什么浓度 2023-07-17 09:59:36
Administrator您好,试剂盒需要根据说明书中标准溶液配制,配制不同浓度标准品做一条标准曲线
说明书里细胞处理的反复冻融2-3次(可在液氮中冻结,37摄氏度水浴中溶解)的处理,可以改为在-80摄氏度的冰箱里冻结,在37摄氏度溶解吗?冻结时间和溶解时间都要求多长啊 2023-06-02 17:30:39
Administrator您好,可以使用-80摄氏度冰箱冻结,冻结时间和溶解时间没有特别要求,反复冻融后细胞能完全破碎就可以。
这个试剂盒测出来的就是还原型GSH的量了是吗?不需要测总GSH和GSSG? 2023-05-23 10:22:20
Administrator您好,这个试剂盒的是样本中还原型谷胱甘肽的含量,不包含氧化型谷胱甘肽含量。
这个试剂盒是适用于所有种属吗? 2022-09-07 20:49:44
Administrator生化试剂盒没有种属限制