L-galactose-1,4-lactone dehydrogenase(Gal LDH) Activity Assay Kit
Cat.No:BC1255 Solarbio
Detection Method:Spectrophotometer/Microplate Reader
Storage:Store at -20℃,6 months
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L-galactose-1,4-lactone dehydrogenase(Gal LDH) Activity Assay KitDetection Method:Spectrophotometer/Microplate Reader
Storage:Store at -20℃,6 months
Qty:
Size:
Name | L-galactose-1,4-lactone dehydrogenase(Gal LDH) Activity Assay Kit |
Detection Method | Spectrophotometer/Microplate Reader |
Storage | Store at -20℃,6 months |
Unit | Kit |
Specification | 100T/96S |
Determination of Significance:
L-galactose way is the key for the synthesis of AsA. Gal LDH is located in the mitochondrial inner membrane and is responsible for the final step of catalyzing the biosynthesis of AsA in plants. Gal LDH is one of the key enzymes in this pathway. It plays an important role in the accumulation of AsA in plants.
Measurement Principle:
Gal LDH catalyzes the reduction of L-galactonolactone to oxidized cytochrome c (Cyt c). Reduced Cyt c has an absorption peak at 550 nm. Measure the increasing rate of reduced Cyt c can calculate the activity of Gal LDH.
Self Provided:
Spectrophotometer/microplate reader, micro glass cuvette/96 well flat-bottom plate, desk centrifuge, adjustable pipette, mortar/homogenizer, ice and distilled water.
Note:Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.
Remark:These protocols are for reference only. Solarbio does not independently validate these methods.
Note:
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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