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Polyamine Oxidase (PAO)Activity Assay Kit

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Polyamine Oxidase (PAO)Activity Assay Kit
Cat.No:
BC5225
Brand:
Solarbio
SKU Tongzhou Beijing Haidian Beijing Wuhan Guangzhou
BC5225-100T/96S Inquiry Inquiry Inquiry Inquiry
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Determination of Significance:

Polyamine Oxidase (PAO) is a key enzyme which catalyse the aerobic degradation of polyamines. Polyamines have strong binding capacity to nucleic acid, protein and cell membrane molecules and directly affect cell growth, differentiation and apoptosis. PAO could be involved in the response to stress and development of plant by regulating polyamines content.

Measurement Principle:

PAO catalyzes polyamines to produce H2O2. Peroxidase catalyzes the production of H2O2 into oxygen and oxidizes o-dianisidine to form colored substance which has light absorption at 500nm. The color depth is linear with PAO activity.

Self Provided:

Spectrophotometer/Microplate reader, balance, constant temperature foster box/water-bath, centrifuge, micro glass cuvette/96-well flat-bottom plate, adjustable transferpettor, mortar/homogenizer/cell ultrasonic crusher, ice and distilled water.

Note:Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.

Remark:
These protocols are for reference only. Solarbio does not independently validate these methods.

Note:
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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