Superoxide Anion Activity Content Assay Kit
Cat.No：BC1295 Solarbio
Detection Method：Spectrophotometer/Microplate Reader
Storage：Store at 2-8℃，6 months
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HIF
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> Biochemical Assay Kits > REDOX Series > Oxidation Series > Superoxide Anion Activity Content Assay Kit
Product Information
| Reagents Required But Not Provided | Please check the manual first, there are some reagents you should prepare by yourself. |
| Name | Superoxide Anion Activity Content Assay Kit |
| Detection Method | Spectrophotometer/Microplate Reader |
| Storage | Store at 2-8℃，6 months |
| Unit | Kit |
| Specification | 100T/96S |
Determination of Significance：
Active oxygen such as superoxide anion in the living body has the functions of immunity and signal transduction. But if it accumulates too much, it will destroy the cell membrane and biomacromolecules, leading to abnormal metabolism of the cells and tissues of the body, and cause many diseases.
Measurement Principle：
The superoxide anion reacts with hydroxylamine hydrochloride to form NO2-, and the NO2- under the action of p-aminobenzenesulfonamide and naphthalene ethylenediamine hydrochloride is produced a red azo compound with a characteristic absorption peak at 530 nm. The content of O2- can be calculated according to the A530 value.
Self Provided：
Spectrophotometer/Microplate reader, water-bath, balance, mortar/homogenizer, centrifuge, micro glass cuvette/96 well flat-bottom plate, chloroform and distilled water.
Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
Experimental Images
Standard Curve for Superoxide Anion Activity Assay Kit
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