Lipid Peroxide (LPO) Content Assay Kit
Cat.No:BC5245 Solarbio
Detection Method:Spectrophotometer/Microplate Reader
Storage:Store at 2-8℃,6 months
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Lipid Peroxide (LPO) Content Assay KitDetection Method:Spectrophotometer/Microplate Reader
Storage:Store at 2-8℃,6 months
Qty:
Size:
Name | Lipid Peroxide (LPO) Content Assay Kit |
Detection Method | Spectrophotometer/Microplate Reader |
Storage | Store at 2-8℃,6 months |
Unit | Kit |
Specification | 100T/96S |
Determination of Significance:
Lipid Hydroperoxide (LPO) is a kind of peroxide produced by unsaturated fatty acid chain through the action of free radicals or reactive oxygen species. Under pathological conditions, the enhancement of lipid peroxidation can lead to the increase of LPO with low content. The increase of LPO content will damage the structure and function of cells. LPO content is closely related to the immune system and aging.
Measurement Principle:
LPO is heated under acidic conditions to produce malondialdehyde (MDA). MDA is condensed with thiobarbituric acid (TBA) to produce a brownish red substance named 3,5,5-trimethyloxazole-2,4-dione. Its maximum absorption wavelength is 532nm. The content of LPO in the sample can be estimated after colorimetry.
Self Provided:
Spectrophotometer/microplate reader, desk centrifuge, adjustable transferpettor, water bath, micro quartz cuvette/96 well flat-bottom UV plate, mortar/homogenizer, ice, distilled water.
Note:Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.
Remark:These protocols are for reference only. Solarbio does not independently validate these methods.
Note:
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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