Storage:Store at 2-8℃,6 months
Determination of Significance:
Hydroxyl radical is a kind of free radical produced by human body in the course of metabolism, which is highly toxic and harmful to organisms. It can cause oxidative damage to carbohydrates, amino acids, proteins and nucleic acids in tissues, leading to cell necrosis or mutation. Hydroxyl radical scavenging capacity is one of the important indicators of antioxidant capacity of samples. It has been widely used in the research of antioxidant health products and medicines.
Measurement Principle:
H2O2/Fe2+ generates hydroxyl radicals through Fenton reaction, and oxidizes Fe2+ into Fe3+ in the aqueous reagent of phenanthroline-Fe2+, resulting in the decreased absorbance of 536 nm, and the inhibition of the decreased rate of absorbance of 536 nm, reflecting the ability of scavenging hydroxyl radicals of samples.
Self Provided:
Spectrophotometer/microplate reader, micro glass cuvette/96 well flat-bottom plate, balance, centrifuge, water bath/constant temperature foster box, transferpettor, mortar/homogenizer, ice and distilled water.
Note:Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.
你好,索莱宝fenton试剂盒的提取液成分是什么,可以用于其他无特殊要求的组织匀浆检测吗,比如说我要做组织MDA的检测,可以用这个提取液进行组织的匀浆吗2022-08-07 14:49:55
Administrator您好,不能用于做组织DNA检测,本身不具备对样品的裂解能力,提取的过程是物理破碎的方式,提取液都是一些缓冲成分和稳定剂,如果提核酸或者提蛋白等要用对应的裂解液而不能用试剂盒中的提取液替代