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Micro Hydroxyl Free Radical Scavenging Capacity Assay Kit

Storage:Store at 2-8℃,6 months

Micro Hydroxyl Free Radical Scavenging Capacity Assay Kit
Cat.No:
BC1325
Brand:
Solarbio

Determination of Significance:

Hydroxyl radical is a kind of free radical produced by human body in the course of metabolism, which is highly toxic and harmful to organisms. It can cause oxidative damage to carbohydrates, amino acids, proteins and nucleic acids in tissues, leading to cell necrosis or mutation. Hydroxyl radical scavenging capacity is one of the important indicators of antioxidant capacity of samples. It has been widely used in the research of antioxidant health products and medicines.

Measurement Principle:

H2O2/Fe2+ generates hydroxyl radicals through Fenton reaction, and oxidizes Fe2+ into Fe3+ in the aqueous reagent of phenanthroline-Fe2+, resulting in the decreased absorbance of 536 nm, and the inhibition of the decreased rate of absorbance of 536 nm, reflecting the ability of scavenging hydroxyl radicals of samples.

Self Provided:

Spectrophotometer/microplate reader, micro glass cuvette/96 well flat-bottom plate, balance, centrifuge, water bath/constant temperature foster box, transferpettor, mortar/homogenizer, ice and distilled water.

Note:Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.

Remark:
These protocols are for reference only. Solarbio does not independently validate these methods.

Note:
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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