Determination of Significance:
Plant phenols have the function of scavenging free radicals, anti-oxidation and anti-aging. It is widely used in cosmetics, food, medicine and other fields because of its high nutritional value and health care function.
Measurement Principle:
In alkaline conditions, phenolic substance reduce tungsten-molybdic acid to form blue compounds which has a absorption peak at 760 nm. The total phenol content of the sample is obtained by measuring the absorbance at 760 nm.
Self Provided:
Spectrophotometer/microplate reader, micro glass cuvette/96 well plate, balance, oven, crusher/mortar, 30-50 sieve, ultrasonic cleaner, centrifuge, 60% alcohol, distilled water.
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