Determination of Significance:
Uric acid is the end product of purine metabolism. Disorders of purine metabolism, energy metabolism and renal excretion of uric acid can cause the increase or decrease of plasma uric acid level. And then lead to a variety of diseases such as ventilation, kidney disease, cardiovascular disease. Therefore, the determination of uric acid content has an important guiding significance in clinical diagnosis.
Measurement Principle:
Uricase can catalyze the decomposition of uric acid into allantoin, CO2 and H2O2. Then Fe2+ in potassium ferrocyanide is oxidized by H2O2 to form Fe3+. Fe3+ can further react with 4-aminoantipyrine and phenol to form red Quinones, which has a characteristic absorption peak at 505 nm. The content of uric acid can be calculated by measuring the absorbance value at 505 nm.
Self Provided:
Spectrophotometer/microplate reader, table centrifuge, water-bath, micro glass cuvette/96 well flat-bottom plate, adjustable pipette, mortar/ homogenizer, EP tube, ice and distilled water.
Note:Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.
请问这款试剂盒可以用于斑马鱼匀浆样品的尿酸含量检测吗2023-05-19 15:08:55
Administrator生化试剂盒没有种属限制,可以检测斑马鱼样本;正式实验之前建议选择 2-3 个预期差异大的样本做预实验。如果样本吸光值不在测量范围内建议稀释或者增加样本量进行检测。
可以检测小鼠血清中尿酸含量吗?2022-10-01 19:15:45
Administrator可以检测,对样本来源种属不做限定的
您好,这款尿酸检测试剂盒所用的微量玻璃比色皿可以用一次性比色皿代替吗?
2022-08-28 09:57:21
Administrator可以